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Effects of Shipping and Heat Stress on Embryonic Mortality in Cattle

02 August 2009

Embryonic loss is the greatest economic loss in the cow/calf industry, affecting number of cows carrying to term and percent calf crop weaned, say Sarah Fields and Dr. George Perry, South Dakota State University Animal and Range Sciences Department.

Fertilization rates are usually between 90 per cent and 100 per cent when semen is present at ovulation. Fertilization usually takes place, but conception rates (number of animals that conceive divided by number of animals inseminated) are usually around 70 per cent for natural service or artificial insemination.

Although nature (poor oocyte quality, disease, chromosomal abnormalities, etc.) contributes much of this loss, management practices can also increase embryonic mortality. Stress, particularly heat and shipping stress, can be detrimental to embryos and decrease pregnancy rates.

Embryonic development

Embryo development begins on day 0, or the day of standing estrus (Table 1). This is the day the female is receptive to the male. Ovulation occurs on day 1 or about 30 hours after the first standing mount. If viable sperm is present, fertilization occurs inside the oviduct shortly after ovulation.

The first cell division occurs on day 2, and by day 3 the embryo has reached the 8-cell stage. Between days 5 and 6 the embryo migrates into the uterine horn and by day 7 to 8 it grows into a blastocyst. At this stage two distinct parts of the embryo can be seen: 1) the inner cell mass, which will form into the fetus and 2) the trophoblast, which will form into the placenta. Between days 9 and 11 the embryo hatches from the zona pellucida, a protective shell that has surrounded the embryo to this point.

On days 15 to 17, the embryo sends a signal to the cow to tell her she is pregnant. This is the first that the cow knows she is pregnant. The embryo attaches to the uterus beginning on day 19, and around day 25, placentation, or cellular interface between the cow and the calf, begins. By day 42 the embryo has fully attached to the uterus of the cow.

Table 1. Time course of early bovine embryo development.
Event Day
Estrus 0
Ovulation 1
Fertilization 1
First cell division 2
8-cell stage 3
Migration to uterus 5-6
Blastocyst 7-8
Hatching 9-11
Maternal recognition of pregnancy 15-17
Attachment to the uterus 19
Adhesion to uterus 21-22
Placentation 25
Definitive attachment of the embryo to the uterus 42
Birth 285

Shipping stress and embryonic mortality

When animals are loaded on a trailer and hauled to a new location, they become stressed and release hormones related to stress. These hormones lead to a release of different hormones that change the uterine environment in which the embryo is developing.

During blastocyst formation, hatching, maternal recognition of pregnancy, and attachment to the uterus, the embryo is vulnerable to these changes. These most critical time points are between days 5 and 42 after insemination (Table 1). Before day 5, the embryo is in the oviduct and is not subject to changes in the uterine environment. Therefore, stress does not influence embryo survivability at this time. The greater the length of time after day 42, the less severe the influence of shipping stress on embryonic loss appears to be. At the time of complete attachment of the embryo to the uterus the embryo is supported by the mother and appears to be not as easily affected by changes in its environment. On the other hand, in between these time points (5–42 days), the embryo is at greatest risk. Shipping during this time can cause detrimental changes to the uterine environment and may result in embryonic mortality.

When should I not ship cows?

Shipping cows between days 5 and 42 causes around a 10 per cent decrease in pregnancy rates (Table 2). Research has also demonstrated that shipping cattle 45 to 60 days after insemination can result in 6 per cent of embryos being lost. Critical time points such as blastocyst formation, hatching, maternal recognition of pregnancy, and adhesion to the uterus take place during this early time of pregnancy.

If any of these time points are disturbed, then the result would lead to increased embryonic mortality and decreased pregnancy rates. Therefore, it is important to plan on transporting cattle before the breeding season or immediately after insemination.

Table 2. Effect of time of transport after insemination on
pregnancy rates.
Days after insemination that transportation occurred
Synchronized pregnancy rate 1 to 4 8 to 12 29 to 33 45 to 60*
per cent pregnancy loss compared
to transportation on days 1 to 4
74 per cent 62 per cent 65 per cent  
  12 per cent 9 per cent 6 per cent*
Breeding season pregnancy rate 95 per cent 94 per cent 94 per cent  
*Loss compared to percent pregnant prior to transportation (pregnancy determined by transrectal ultrasonography).
Data adapted from Harrington et al. 1995 and Merrill et al. 2007.

When can I ship cows?

Shipping between days 1–4 is best. The embryo is still in the oviduct during this time; therefore, it is likely not subjected to uterine changes. After day 45, the embryo is well established and fully attached with the placenta; therefore, it is less susceptible to the changes resulting from stress. Shipping at this point is less risky. However, embryonic loss from shipping has been reported up to 60 days after insemination.

Care should always be taken to reduce the stress involved when animals are shipped. Do not overcrowd trailers, and handle cattle as gently and calmly as possible.

Table 3. Time points for shipping pregnant cattle.
Day
When to ship 1 _ 4 or after 45 to 60
When not to ship 6 _ 42

Heat stress and embryonic mortality

The best time to ship cattle is during early stages of development. However, this is also the time when the embryo is most susceptible to increased temperatures.

Temperature, humidity, radiant heat, and wind all affect heat stress in cows. The rectal temperature of cattle is normally 102.2°F, and an increase in rectal temperature by as little as 2°F can result in decreased embryonic development. When rectal temperatures reach 105.8°F for as little as 9 hours on the day of insemination, embryonic development can be compromised. Heat stress has also been shown to change follicular waves, resulting in reduced oocyte quality. Researchers have shown that heat stress 42 days prior up to 40 days after breeding can affect pregnancy rates. This illustrates how important it is to plan ahead for the breeding season.

Shade, fans, and misters can all reduce the effects of heat stress in natural service or artificial insemination (AI) programs. In humid areas, misters may not actually benefit the animals. If the water cannot evaporate, it will not be effective at cooling the animal.

Producers that utilize AI can also implement timed AI (TAI) protocols to increase pregnancy rates during the hot summer months.

Timed AI has increased pregnancy rates over animals inseminated 12 hours after estrus detection and under heat stress. This is most likely because fewer animals show signs of estrus under heat stress. When the weather is too hot, animals tend not to move around as much and do not show signs of standing estrus.

Heat detection is a vital part of getting more animals pregnant. Since fewer animals are seen in heat, fewer animals can be inseminated. In this case, TAI would be the best protocol to use because it eliminates heat detection.

Using embryo transfer during times of heat stress can also increase pregnancy rates. High quality, fresh embryos have been proven to increase pregnancy rates over AI in heat stressed cows. Embryos at time of embryo transfer can adapt to the elevated temperatures.

Conclusion

Getting cows/heifers pregnant during the breeding season, especially early in the breeding season, can have a tremendous impact on the profitability of a cow/calf operation. Large amounts of time, effort, and costs are required to have a successful breeding season (natural service or AI). Stressing animals during critical time points of embryo development can have a serious negative impact on pregnancy rates. Heat stress can decrease pregnancy rates during early embryonic development, and stress from shipping can also decrease pregnancy rates throughout early embryo development. Therefore, planning around the breeding season becomes an important management tool for maximizing pregnancy success.

June 2009

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